Eleni Douni Research Group

Ongoing and completed Dissertations and PhD Theses in our lab:

Completed Ph.D. projects

Anthi Kolokotroni, 2023, PhD thesis title: «Study of the pathophysiological role of RANKL protein in the mammary gland of transgenic mice». Department of Biotechnology, Agricultural University of Athens (from 2017). Supervisor Professor: E Douni.

Vassiliki-Iris Perivolidi, 2023, PhD thesis title "Studying pathophysiological mechanisms in modeled SLC25A46-mediated neurological disease in mice through Molecular Genetics and Proteomic approaches". Department of Biotechnology, Agricultural University of Athens (from 2018). Supervisor Professor: E Douni.

Maria Papadaki, 2019, PhD thesis title: "Studying the role of RANKL in osteoimmune interactions using transgenic mouse models of bone resorption", Department of Biotechnology, Agricultural University of Athens. Supervisor Professor: E Douni.

Foteini Violitzi, 2019, PhD thesis title: "Study of the pathophysiological role of DnaJC11 gene in a mouse model of neuromuscular disease". Department of Biotechnology, Agricultural University of Athens, Supervisor Professor: E Douni.

Maria Eirini Terzenidou, 2017, PhD thesis title: “Studying the pathogenic role of the mitochondrial carrier SLC25 in a genetic model of neurological disease in mice”. Department of Biotechnology, Agricultural University of Athens. Supervisor Professor: E Douni.

Niti Alexandra, 2015, PhD thesis title: “Studying the pathogenic role of RANKL in animal models”. Medical School, University of Athens. Supervisor Professor: M Koutsilieris

Ioakeimidis Fotios, 2014, PhD thesis title: “Studying the pathogenic mechanisms in a novel autosomal recessive neuromuscular mouse model using phenotypic, genetic and molecular analyses». Department of Biology, Aristotle University of Thessaloniki. Supervisor Professor: A Lazou. 

Vagelis Rinotas, 2014, PhD thesis title: "Study of RANKL-mediated pathogenic mechanisms in animal models and new therapeutic approaches». Department of Biotechnology, Agricultural University of Athens. Supervisor Professor: E Douni 

Ongoing Ph.D. projects

Christos Chrysovalantis Syrigos, PhD thesis title "Studying the role of RANKL in carcinogenesis and metastasis using transgenic mice", Department of Biotechnology, Agricultural University of Athens (since 2023). Supervisor Professor: E Douni.

Elisavet Ioannidou, PhD thesis title "Study of molecular and epigenetic mechanisms in a transgenic mouse model of RANKL-mediated osteoporosis", Department of Biotechnology, Agricultural University of Athens (since 2021). Supervisor Professor: E Douni.

Evi Gkikopoulou, PhD thesis title "Studying the role of RANKL protein in mammary cancer and bone metastasis in transgenic mice".  Department of Biotechnology, Agricultural University of Athens (from 2019). Supervisor Professor: E Douni.

Completed MSc Dissertations (Supervisor)

Elisavet Ioannidou, 2021, MSc Dissertation, title: "Αnalysis of the transcriptome in modeled osteoporosis", Medical School, University of Athens, MSc program "Molecular Biomedicine".

Polina Pantelidou, 2019, MSc Dissertation, title: "Investigating the role of exosomes in osteoporotic models", Department of Pharmacy, University of Athens,  MSc program "Nanomedicine".

Ippokratis Lygizos, 2018, MSc Dissertation, title: "Study of SLC25A46 deletion in the mouse nervous system". Department of Biotechnology, Agricultural University of Athens.

Eirini Gialitaki, 2015, «Identification of polymorphisms in the DNAJC11 gene in ALS patients». Department of Biotechnology, Agricultural University of Athens. 

Toshimi Kano, 2013, MSc Dissertation, title: «Molecular and Genetic analysis of the Slc25 gene in transgenic mice». Department of Biotechnology, Agricultural University of Athens. 

Foteini Violitzi, 2013, MSc Dissertation, title: «Evaluation of novel RANKL inhibitors in cellular assays for the identification of novel therapeutics in osteoporosis». Department of Biotechnology, Agricultural University of Athens. 

Niti Alexandra, 2010, MSc Dissertation, title: “Studying the overxpression of RANKL in transgenic mice: Generation of a novel model for osteoporosis?”. Medical School, University of Athens.

Karakostas Alexandros, 2010, MSc Dissertation, title: “Detailed genetic analysis in mouse chromosome 18 for the identification of a novel pathogenic target causing lethal neurological phenotype”. Department of Agricultural Biotechnology, Agricultural University of Athens.

Completed BSc Projects (Supervisor)

Andrikopoulou Dimitra, 2024, Final year dissertation, title: "Study of the transcriptome in the TgRANKL transgenic model of osteoporosis". Department of Biotechnology, Agricultural University of Athens.

Chryssa-Eleni Petraki, 2024, Final year dissertation, title: "Study of the effect of anti-osteoporotic drugs in a model of bone metastasis in transgenic TgRANKL mice". Department of Biotechnology, Agricultural University of Athens.

Panagiotis Theofilidis, 2023, Final year dissertation, title: "Confirmation and analysis of RNA sequencing results in the TgRANKL transgenic model of osteoporosis". Department of Biotechnology, Agricultural University of Athens.

Andreas Athanasios Tzotzos Demak, 2023, Final year dissertation, title: "Study of the lack of SLC25A46 protein in an induced silencing cell system and in the mouse cerebellum". Department of Biotechnology, Agricultural University of Athens.

Ioanna Mantogiannakou, 2023, Final year dissertation, title: "Study of breast cancer metastases in the TgRANKL transgenic model of osteoporosis". Co-supervision with Prof. R. Tsitsiloni, Department of Biology, University of Athens.

Nikolaos Terzoglou, 2023, Final year dissertation, title: "Characterization of the transgenic line of Tg5520 mice for expression of the SLC25A46-FLAG transgene". Department of Biotechnology, Agricultural University of Athens.

Sofia Schoina, 2023, Final year dissertation, title: "Study of the effect of RANKL protein on breast cancer cells through proteomic analysis". Department of Biotechnology, Agricultural University of Athens.

Theodora Giannousa, 2023, Final year dissertation, title: "Study of the effect of RANKL protein on protein phosphorylation in breast cancer cells". Department of Biotechnology, Agricultural University of Athens.

Pavlina-Iliana Moutzouri, 2023, Final year dissertation, title: "Study of mitochondrial function in the absence of the SLC25A46 protein in an induced silencing cell system and in the mouse". Department of Biotechnology, Agricultural University of Athens.

Maria Skalioti, 2023, Final year dissertation, title: "Study of gene expression profiling in transgenic model of RANKL-induced osteoporosis". Department of Biotechnology, Agricultural University of Athens.

Vassiliki Kontou, 2022, Final year dissertation, title: "Detection of protein interaction network of SLC25A46 protein in thymus and heart of transgenic SLC25A46-FLAG mice". Department of Biotechnology, Agricultural University of Athens.

Vassiliki Geronta, 2022, Final year dissertation, title: "Comparative expression analysis of a transgenic model of RANKL-induced breast cancer". Department of Biotechnology, Agricultural University of Athens.

Marios Itsios, 2022, Final year dissertation, title: "Comparative expression analysis in the TgRANKL transgenic model of osteoporosis". Department of Biotechnology, Agricultural University of Athens.

Ioanna Mina, 2022, Final year dissertation, title: "Study of the mammary gland during lactation in TgRANKL transgenic mice". Department of Biotechnology, Agricultural University of Athens.

Antonios Papoutsis, 2022, Final year dissertation, title: "Study of the expression of mammary stem cell markers in TgRANKL transgenic mice". Department of Biotechnology, Agricultural University of Athens.

Christos Chrysovalantis Syrigos, 2022, Final year dissertation, title: "Study of the effect of RANKL protein on breast cancer cells and mammary glands of TgRANKL transgenic mice". Department of Biotechnology, Agricultural University of Athens.

Nefeli Kammenou, 2021, Final year dissertation, title: "Study of the development of the mammary gland during pregnancy in TgRANKL transgenic mice". Department of Biotechnology, Agricultural University of Athens.

Irene Papaioannou, 2021, Final year dissertation, title: "Characterization of the transgenic line Tg5537 as regards the expression of the transgene SLC25A46-FLAG". Department of Biotechnology, Agricultural University of Athens.

Nikolaos Kontopoulos, 2021, Final year dissertation, title: "Study of the mechanisms of bone loss in animal models of mitochondrial neurological diseases". Department of Biotechnology, Agricultural University of Athens.

Dimitra Douskou, 2021, Final year dissertation, title: "Molecular and imaging study of anti-osteoporotic therapies in TgRANKL transgenic models of osteopororsis". Co-supervision with Prof. R. Tsitsiloni, Department of Biology, University of Athens.

Marina Boltsi, 2021, Final year dissertation, title: "Detection of SLC25A46 Protein Interactions in the Central Nervous System of SLC25A46-FLAG Transgenic Mice". Department of Biotechnology, Agricultural University of Athens.

Eleni Dermitzaki, 2020, Final year dissertation, title: "Molecular and imaging analysis of the mammary gland in the transgenic mouse model of osteoporosis Tg5516". Department of Biotechnology, Agricultural University of Athens.

Apostolos Papadopoulos, 2020, Final year dissertation, title: "Analysis of the musculoskeletal system of ΤgRANKL transgenic mice". Department of Biotechnology, Agricultural University of Athens. 

Panagiotis Nikolaou, 2020, Final year dissertation, title: "Comparative analysis of femurs from TgRANKL transgenic mouse models of osteoporosis". Department of Biotechnology, Agricultural University of Athens. 

Ioannis Ntounias, 2020, Final year dissertation, title: "Generation of recombinant plasmids for the human SLC25A46 gene". Department of Biotechnology, Agricultural University of Athens. 

Athanasios Rentis, 2020, Final year dissertation, title: "Regulation of the mammary glands from ΤgRANKL transgenic mice with progesterone". Department of Biotechnology, Agricultural University of Athens. 

Eirini Efstathiou, 2019, Final year dissertation, title: " Molecular and imaging analysis of the mammary gland from the transgenic mouse model Τg5519". Department of Biotechnology, Agricultural University of Athens. 

Konstantina-Maria Nikolaou, 2019, Final year dissertation, title: "Analysis of the skeletal system of mouse models with mitochondrial neurological diseases". Department of Biotechnology, Agricultural University of Athens. 

Aristofanis Stavrou, 2019, Final year dissertation, title: "Generation of recombinant plasmids for the human mutations of SLC25A46 gene". Department of Biotechnology, Agricultural University of Athens. 

Konstantinos Kritikos, 2019, Final year dissertation, title: "Analysis of mesenchymal bone marrow cells from transgenic models of osteoporosis". Department of Biotechnology, Agricultural University of Athens. 

Christos Mantzoros, 2019, Final year dissertation, title: "Mapping of the SLC25A46 protein regions involved in mitochondrial localization". Department of Biotechnology, Agricultural University of Athens. 

Evi Gkikopoulou, 2019, Final year dissertation, title: "Analysis of the mammary gland of TgRANKL transgenic mice". Department of Biotechnology, Agricultural University of Athens. 

Georgia Daraki, 2019, Final year dissertation, title: "Generation of recombinant plasmids for the expression of the chimeric protein SLC25A46-FLAG in mammalian cells". Department of Biotechnology, Agricultural University of Athens. 

Efthimios Tzortzis, 2019, Final year dissertation, title: "Study of bone marrow adipogenesis mechanisms in the transgenic osteoporosis model Τg5519". Department of Biotechnology, Agricultural University of Athens. 

Panagiota Siatra, 2018, Final year dissertation, title: "Study of bone marrow adipogenesis formation in the transgenic osteoporosis model Τg5516. Department of Biotechnology, Agricultural University of Athens. 

Emmanouil Siniorakis, 2018, Final year dissertation, title: "Study of bone marrow adipogenesis in transgenic models of osteoporosis". Department of Biotechnology, Agricultural University of Athens.

Antigone Kontou, 2017, Final year dissertation, title: : "Differentiation potential of mesenchymal stromal cells in TgRANKL mouse models of osteoporosis". Department of Biosciences and Biotechnology, University of Camerino, Italy.

Vassilios Bakogiannis, 2017, Final year dissertation, title: "Effect of Tumor Necrosis Factor (TNF) deletion in ΤgRANKL transgenic models of osteoporosis". Department of Biotechnology, Agricultural University of Athens. 

Vassiliki-Iris Perivolidi, 2017, Final year dissertation, title: "Detection of DNAJC11 protein interactions in  transgenic  DNAJC11-FLAG mice". Department of Biotechnology, Agricultural University of Athens.

Cristina Fracassi, 2016, Final year dissertation, title: "Approaches for analysis of Bone Marrow Adiposity in a mouse model of osteoporosis". Department of Biosciences and Biotechnology, University of Camerino, Italy.

Theodora Manolakou, 2016, «Knocking out DnaJC11 gene in a cell line through CRISPR/CAS9». Department of Biotechnology, Agricultural University of Athens.

Anastasia Rigou, 2016, «Characterization of DnaJC11-FLAG transgenic mice». Department of Biotechnology, Agricultural University of Athens.

Niki Chalkidi, 2015, Final year dissertation, title: «Evaluation of small molecules inhibitors of RANKL in cellular assays». Department of Biotechnology, Agricultural University of Athens.

Marios-Evangelos Boyongo, 2014, Final year dissertation, title: «Study of the interactions of DNAJC11 protein with other proteins». Department of Biotechnology, Agricultural University of Athens. 

Toshimi Kano, 2012, Final year dissertation, title: “Study of the expression of the SLC25 gene in a mouse model of recessive neurological disease”. Department of Agricultural Biotechnology, Agricultural University of Athens.

Foteini Violitzi, 2012, Final year dissertation, title: «Inhibition of trimer formation in BAFF through site-directed mutagenesis». Department of Agricultural Biotechnology, Agricultural University of Athens. 

Ioakeimidis Fotios, 2008, Final year dissertation, title: “Studying the role of the cytoplasmic region of TNF in transgenic mice”. Department of Agricultural Biotechnology, Agricultural University of Athens.

Karakostas Alexandros, 2008, Final year dissertation, title: “Genetic analysis of a novel model of ataxia”. Department of Agricultural Biotechnology, Agricultural University of Athens.

Mermelekas George, 2005, Final year dissertation, title: “Genetic analysis of animal models of human diseases using polymorphic markers”. Department of Biological applications, University of Ioannina.