Cryorederivation

Information

Our Facility generates live mice by performing surgical transfer of embryos recovered from a cryopreserved stock (kept into our CryoBank or imported). Embryo transfer has been demonstrated to be an effective method in order to reconstitute a pathogen-free mouse colony. The zona pellucida of embryos provides an effective barrier for pathogens.

Cryopreserved embryos

If the cryopreserved line belongs to our Cryobank, 40-60 frozen embryos are thawed, recovered and transferred into pseudopregnant females. Upon births, we provide toe biopsies to the investigators for following screening.
For lines to be imported the Facility arranges all transfer details, receives the embryos and performs the aforementioned procedure to revive the requested mouse line.